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| 产品名称： | MUG-CHOR-1 |
|---|---|
| 商品货号： | TS260252 |
| <strong>Cell line </strong>： | MUG-CHOR-1 |
| DSMZ no. ： | TS260252 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chordoma |
| Origin ： | established in 2006 from a recurrent morphologically "classic" sacrococcygeal chordoma of a 58-year-old Caucasian woman |
| Reference(s) ： | 18729 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers; image |
| Medium ： | 80% mixture of Iscoves MDM and RPMI 1640 (at 5:1) + 20% h.i. FBS + 1% insulin-transferrin-sodium selenite (IST) + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 75-100 hours |
| Harvest ： | cell harvest of ca. 4 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |