微信小李
微信小章
产品简介
购买须知
| 产品名称： | PACADD-188 |
|---|---|
| 商品货号： | TS260254 |
| <strong>Cell line </strong>： | PACADD-188 |
| DSMZ no. ： | TS260254 |
| Species ： | human (Homo sapiens) |
| Cell type ： | pancreas carcinoma |
| Origin ： | established in 2011 from the intraepithelial neoplasia of a 68-year-old woman with a ductal adenocarcinoma of the pancreas (stage classification pT3N1M0/G3) |
| Reference(s) ： | 17618 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing adherently in monolayers in tight islands |
| Medium ： | 80% mixture of DMEM and Keratinocyte SFM (at 1:1) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 once a week using trypsin/EDTA for about 10 min. to detach cells |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2-4 days |
| Harvest ： | cell harvest of ca. 8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |