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| 产品名称： | R-06E |
|---|---|
| 商品货号： | TS260256 |
| <strong>Cell line </strong>： | R-06E |
| DSMZ no. ： | TS260256 |
| Species ： | confirmed Rousettus aegyptiacus origin by COI DNA Barcoding |
| Cell type ： | embryonal |
| Origin ： | established from body cells of the fetus from Rousettus aegyptiacus (fruit bat); primary cells were immortalized by transfection with an expression plasmid for E1A and E1B open reading frames of human adenovirus 5 in non-cognate expression cassettes; cells are described in the literature to be permissive for the attenuated poxvirus ´modified vaccinia Ankara´ |
| Reference(s) ： | 18014 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The cell line was established from animals of the captive breeding of a zoo; Additionally to our own virus testing for human pathogenic viruses, the animals and the cells were shown to be negative for rabies and henipaviruses (incl. Hendra, Nipah and Cedar virus) by external laboratories. The cells were categorized biosafety level 1. |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small fibroblast-like adherent cells growing as monolayer; image; image |
| Medium ： | 95% mixture of DMEM and Ham´s F12 (at 1:1) + 5% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:10 - 1:20 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 18 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II - |