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| 产品名称： | SK-MM-1 |
|---|---|
| 商品货号： | TS260263 |
| <strong>Cell line </strong>： | SK-MM-1 |
| DSMZ no. ： | TS260263 |
| Species ： | human (Homo sapiens) |
| Cell type ： | plasma cell leukemia |
| Origin ： | established in 1981 from the bone marrow of a 55-year-old man with plasma cell leukemia at relapse after chemotherapy; cells are described in the literature to carry the t(6;14) effecting rearrangement of the IRF4 (MUM1) and IGH genes and t(14;20) leading to juxtaposition of IGH to MAFB |
| Reference(s) ： | 14521, 18115, 18116 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing in suspension singly or in clusters; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1.5 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 1.8 - 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD21 -, CD30 +, CD34 -, CD80 -, CD138 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Cytogenetics ： | human flat moded hypodiploid karyotype; 41-45<2n>X, -Y, +1, -13, dic(1;13)(p13.2;p13), t(4;19)(q11;p11), del(6)(q25), der(6)t(6;14)(p25;q32), der(8)t(8;14)(q24;q13)inv(14)(q13q32)t(14;6)(q32;p25)x2, der(14)t(8;14)(q24;q32)t(14;20)(q32;q11), der(15)t(11;15)(q12;p11), der(20)t(14;20); resembles published karyotype; carries concurrent t(6;14) and t(14;20) respectively rearranging IGH with IRF4 (MUM1) and MAFB plus MYC |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |