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| 产品名称： | HG-3 |
|---|---|
| 商品货号： | TS260271 |
| <strong>Cell line </strong>： | HG-3 |
| DSMZ no. ： | TS260271 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1998 from leukemic B-cells of a 70-year-old Caucasian man with chronic lymphocytic leukemia at diagnosis (CLL, Rai stage II). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 19172, 19507, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing partly in clumps in suspension and slightly adherent; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days; starting in a 12- or 24-well plate could be of advantage |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD4 -, CD5 +, CD10 -, CD13 -, CD15 +, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Cytogenetics ： | human near-diploid karyotype; 46(44-46)<2n>XY, der(10)t(10;11)(q26;q24.1), del(13)(q12q32); carries del(13) associated with CLL; marginal resemblance to original karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |