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| 产品名称： | I83-LCL |
|---|---|
| 商品货号： | TS260272 |
| <strong>Cell line </strong>： | I83-LCL |
| DSMZ no. ： | TS260272 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B lymphoblastoid cells (from patient with chronic B cell leukemia) |
| Origin ： | normal B-cell derived lymphoblastoid cell line established in 1994 from a 71-year-old Caucasian man with chronic lymphocytic leukemia (CLL); autologous to cell line I83-E95 (DSM ACC 763) |
| Reference(s) ： | 19334, 19172 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing mainly in clumps in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.4-1 x 106 cells/ml; optimal split ratio 1:2 - 1:4 every 2-4 days; split culture or change medium once or twice a week to eliminate a lot of cell debris |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximum density at ca. 1.3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; in the referenced paper, this cell line was described as IgM +, lambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed an authentic STR DNA profile as I83-E95 (ACC 763) |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype; 83-92<4n>XXYY, -2, -6, +10, +10, -16, der(10)t(2;10)(q21.2;q21) |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |