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| 产品名称： | I83-E95 |
|---|---|
| 商品货号： | TS260273 |
| <strong>Cell line </strong>： | I83-E95 |
| DSMZ no. ： | TS260273 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1994 from the peripheral blood of a 75-year-old Caucasian man with chronic lymphocytic leukemia (CLL, Binet A stage at diagnosis) and subjected to immortalization by Epstein-Barr virus; EBV+ B-lymphoblastoid sister cell line (B-LCL) I83-LCL is available (ACC 764) |
| Reference(s) ： | 19334, 19172 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for HHV-4 (EBV) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small to medium sized irregularly shaped cells growing singly or in clumps |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml in 24-well plates; optimal split ratio 1:2 - 1:3 every 2-3 days; cells grow better in 24-well plates than in culture flasks |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-60 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD4 +, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed an STR DNA profile matching sister cell line I83-LCL (ACC 764) |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype; 83-88<4n>XXYY, -6, -14, -18, der(2)t(2;11)(q12;q25)x2, der(11)t(2;11)(q12;q13)x2, der(13)t(13;13)(p11;q14)x2, der(14)t(14;14)(q32;q22)x2, der(22)t(13;22)(q31;p11)x2; carries chromosome rearrangements leading to loss of 13q11-14; resembles published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |