微信小李
微信小章
产品简介
购买须知
| 产品名称： | HAIR-M |
|---|---|
| 商品货号： | TS260276 |
| <strong>Cell line </strong>： | HAIR-M |
| DSMZ no. ： | TS260276 |
| Species ： | human (Homo sapiens) |
| Cell type ： | hairy cell leukemia |
| Origin ： | established in 1981 from the peripheral blood of an 86-year-old Japanese man with hairy cell leukemia. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 19373, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid cells growing as single cells or in clumps in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml in 24-well plates; optimal split ratio 1:2 - 1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD71 +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG +, sm/IgM - sm/cykappa +, sm/lambda -; image |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype 84-96<4n>XXYY, +2, -6, +9, +14, -19, -19, der(X)t(X;21)(p22;q22), der(Y)t(Y;19)(p11.3;q11)x2, der(17)t(11;17)(q11;p11)x2, der(17)t(17;22)(p11;q11), der(18)t(16;18)(q11;p11.3)x2-3; Subclones with additional numerical and structural alterations: e.g. t(15;17)(q25;q23)der(17)t(17;22)(p11;q11) resembles published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -; NGS: EBV + |