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| 产品名称： | U-2904 |
|---|---|
| 商品货号： | TS260278 |
| <strong>Cell line </strong>： | U-2904 |
| DSMZ no. ： | TS260278 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1991 from the pleural effusion of a 59-year-old man with centroblastic-centrocytic B-cell non-Hodgkin-lymphoma at diagnosis that secondarily transformed to centroblastic lymphoma after radiotherapy; cells were harvested after chemotherapy; described in the literature to carry t(8;14) and t(14;18) affecting MYC-IGH and IGH-BCL2, respectively |
| Reference(s) ： | 18114 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.7 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every second day; maintain at ca. 0.5-2.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25 - 35 hours |
| Harvest ： | maximum density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Cytogenetics ： | human flat-moded near-diploid karyotype; 43-47<2n>X-Y, +4, -13, +20, der(1)t(1;22)(p11;q11), inv(1)(q11q21), del(6)(q11), t(8;14)(q24;q32), der(10)t(10;13)(p13;q13), t(14;18)(q32;q21), del(16)(q22); carries biallelic rearrangements of IGH with MYC and BCL2, respectively; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |