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| 产品名称： | WA-C3CD5+ |
|---|---|
| 商品货号： | TS260280 |
| <strong>Cell line </strong>： | WA-C3CD5+ |
| DSMZ no. ： | TS260280 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1994 from the peripheral blood leukocytes of a Caucasian man with B-type chronic lymphocytic leukemia (CLL, Rai stage I at diagnosis); CD5 positive sister cell line of WA-OSEL (DSM ACC 767) |
| Reference(s) ： | 19334, 19172 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for HHV-4 (EBV) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly and in small clusters in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in 24-well plates; optimal split ratio 1:2 - 1:3 every two days; maintain at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 - 50 hours |
| Harvest ： | maximum density at ca. 0.7-1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 +, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed an authentic STR DNA profile as WA-OSEL (ACC 767) |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 6% polyploidy;43-51<2n>XY, +9, +13, +21, der(6)t(6;9)(q14;q21), der(7)t(7;17)(q10;q10), del(13)(q14q21)x2, del(13)(q14.2q14.3); nullisomy for DLEU2 locus via focal chr. 13 deletion associated with CLL; marginally related to published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |