微信小李
微信小章
产品简介
购买须知
| 产品名称： | WA-OSEL |
|---|---|
| 商品货号： | TS260281 |
| <strong>Cell line </strong>： | WA-OSEL |
| DSMZ no. ： | TS260281 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1993 from a peripheral blood sample of a Caucasian man with B-type chronic lymphocytic leukemia; CD5 negative sister cell line of WA-C3CD5+ (DSM ACC 769) |
| Reference(s) ： | 19334, 19172 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension, partly aggregating or slightly adherent |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.2 x 106 cells/ml; optimal split ratio 1:3 - 1:5 twice a week; maintain at ca. 0.5-1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximum density at ca. 1.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 -, CD10 -, CD13 -, CD14 -, CD19 +, CD20 +, CD34 -, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa+, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed an authentic STR DNA profile as WA-C3CD5+ (ACC 769) |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype; 83-92<4n>XXYY, -6, -17, -18, del(11)(q22)x1-2, i(17)(q10) |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |