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| 产品名称： | UOC-M1 |
|---|---|
| 商品货号： | TS260291 |
| <strong>Cell line </strong>： | UOC-M1 |
| DSMZ no. ： | TS260291 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established at diagnosis in 1992 from the malignant bone marrow cells of a 68-year-old man with undifferentiated acute myeloid leukemia (FAB AML M1) of the megakaryoblastic lineage; cells are described in the literature to carry four copies of the KMT2A (MLL) gene |
| Reference(s) ： | 19887 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to elongated cells growing singly in suspension and some cells adherently; image; image |
| Medium ： | 90% McCoy´s 5a + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:2 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; adherent cells can be detached by tapping or using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximum density at ca. 1.5-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD4 -, CD7 +, CD13 +, CD14 -, CD15 -, CD19 -, CD24 +, CD33 +, CD34 +, CD38 +, CD41 +, CD42 -, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Cytogenetics ： | human flat-moded hypodiploid karyotype; -39-44<2n>XY, -7, -9, -11, -14, -19, +21, +21, der(Y)t(Y;9)(p11;p13)t(9;Y)(p24;p11), del(5)(q14q35), der(5)t(Y;5)(q11;p15)t(Y;9)(q12;q11), der(9;11)(11qter->q11::19p1?->p1?::9pter->q11::19p1?->p1?::11q11->q25::11q14->qter), der(17)t(7;17)(p15;p13), r(19)(p13q11), der(21)t(16;21)(p11;p11), der(21t(19;21)(p11;p11), der(21)trp(21)(q21.2q22.3)t(11;21)(q22;q22.3); copy number increase (to four per diploid genome) of unrearranged KMT2A (MLL) locus; resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |