微信小李
微信小章
产品简介
购买须知
| 产品名称： | CII |
|---|---|
| 商品货号： | TS260292 |
| <strong>Cell line </strong>： | CII |
| DSMZ no. ： | TS260292 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1979 from Epstein-Barr virus (EBV) immortalized neoplastic lymphocytes of a 47-year-old woman with chronic lymphocytic leukemia (CLL, Rai II stage at diagnosis); the CI cell line - formerly thought of as a B-lymphoblastoid sister - is now deemed a subclone of the putatively oligoclonal CII cell line; cells express stereotyped subset 5 BCR |
| Reference(s) ： | 19602, 19603, 19604, 19172 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | semi-adherent cells show round or ovoid morphology growing in clusters; long cultivation period leads to attachment of the cells and epitheloid morphology; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/25 cm2; optimal split ratio 1:2 - 1:4 every 2-4 days; adherent cells can be detached by tapping |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | cell harvest of ca. 4 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed authentic STR DNA profile identical to CI |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype; 93-97<4n>XXXXX, +X, +12, der(1)t(1;20)(p36;p11), der(12)t(12;14)(p11;q11), add(17)(p11)x2, del(16)(q12); sdl with additional numerical and structural aberrations; some resemblance to published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |