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| 产品名称： | 232A4 |
|---|---|
| 商品货号： | TS260293 |
| <strong>Cell line </strong>： | 232A4 |
| DSMZ no. ： | TS260293 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B lymphoblastoid cells |
| Origin ： | established from Epstein-Barr virus (EBV) immortalized normal B-cells of a man with chronic lymphocytic leukemia (CLL, Rai 0 stage at diagnosis) |
| Reference(s) ： | 18113, 19334, 19172 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for HHV-4 (EBV) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells, growing singly or in clusters in suspension; the culture contains usually a large amount of cell debris |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; optimal split saturated culture 1:2 - 1:4 every 2-4 days; the culture usually contains a significant amount of cell debris |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at ca. 1.5-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cyIgM -, cykappa +, cylambda -; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Cytogenetics ： | human near diploid karyotype with 4% polyploidy; 46(43-46)<2n>XY, no consistent abnormality detected; matches published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |