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| 产品名称： | L4.5 |
|---|---|
| 商品货号： | TS260295 |
| <strong>Cell line </strong>： | L4.5 |
| DSMZ no. ： | TS260295 |
| Species ： | confirmed as mus musculus by COI DNA barcoding |
| Cell type ： | derivative of L-929 |
| Origin ： | derived from L-929 cells by transfection with vector pTEJ-8 (mediating geneticin-resistance) containing the cDNA for murine CD40L in 1996; cells are described in the literature to express murine CD154 |
| Reference(s) ： | 19896, 19897, 19898, 19899 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The cell line L4.5 derived from L-929 cells by transfection with the geneticin-resistance mediating vector pTEJ-8 containing the cDNA for murine CD40L and thus has to be regarded as genetically modified organism (GMO). Neither the parental cell line nor the plasmid and its expression products are harmful to man. Hence, the cell line is categorized risk group 1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid cells growing as monolayer |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:10 - 1:20 once or twice a week using trypsin without EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |