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| 产品名称： | LOPRA-1 |
|---|---|
| 商品货号： | TS260300 |
| <strong>Cell line </strong>： | LOPRA-1 |
| DSMZ no. ： | TS260300 |
| Species ： | human (Homo sapiens) |
| Cell type ： | multiple myeloma |
| Origin ： | established in 1982 from ascites fluid containing malignant plasma cells of a 62-year-old woman with fatal multiple myeloma (stage IIA, IgA2/kappa) after chemotherapy; described in the literature to be growth-dependent on IL-6 |
| Reference(s) ： | 18122 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | variously sized round to orthogonal cells growing in suspension and occasionally in small clumps |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 10 ng/ml human IL-6 |
| Subculture ： | split saturated culture 1:2 every 4-6 days; seed out at ca. 1 x 106 cells/well in 24-well-plates; cultivate cells initially in 24-well-plates after recovery, but cells can be grown subsequently in flasks; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 5 days |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD28 +, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |