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| 产品名称： | HC-11 |
|---|---|
| 商品货号： | TS260301 |
| <strong>Cell line </strong>： | HC-11 |
| DSMZ no. ： | TS260301 |
| Species ： | confirmed as mouse by animal-PCR and as mus musculus by COI DNA barcoding |
| Cell type ： | normal mammary epithelial |
| Origin ： | derived in 1987 as a prolactin-responsive subclone of the COMMA-1D cell line which was established from normal mammary epithelial cells of an adult female BALB/c mouse |
| Reference(s) ： | 24247 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid adherent cells growing as monolayer |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 5 µg/ml insulin (bovine or human) + 10 ng/ml EGF + 4 mM L-glutamine |
| Subculture ： | seed out at ca. 0.3-1 x 106 cells/25 cm2 flask; split confluent culture 1:2 - 1:4 every 2-4 days using trypsin/EDTA for about 10 min |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-80 hours |
| Harvest ： | cell harvest of ca. 6 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |