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| 产品名称： | MOLM-14 |
|---|---|
| 商品货号： | TS260302 |
| <strong>Cell line </strong>： | MOLM-14 |
| DSMZ no. ： | TS260302 |
| Species ： | human (Homo sapiens) |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndrome (MDS, refractory anemia with excess of blasts, RAEB); carries internal tandem duplication of FLT3; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-13 (ACC 554) |
| Reference(s) ： | 14869, 14743, 16558 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly single round cells growing in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml in 24-well-plates; maintain at ca 0.5-1.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile identical to sister cell line MOLM-13 by a search of the international STR database |
| Cytogenetics ： | human hyperdiploid karyotype; -49(46-50)<2n>XY, +6, +8, +13, der(2)t(1;2)(q31;q35), ins(11;9)(q23;p22p23), del(14(q23q32.3), del(16)(q11.2q13.1); sdl without der(2) but with tetrasomy 8 and/or i(8)(q10); carries insertional variant of t(9;11) recurrent in AML, effecting rearrangement of KMT2A (MLL) with MLLT3 (AF9); ins(11;9) plus trisomies 8 and 13 also present in sister cell line MOLM-13 which it closely resembles |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |