微信小李
微信小章
产品简介
购买须知
| 产品名称： | WSU-DLCL |
|---|---|
| 商品货号： | TS260303 |
| <strong>Cell line </strong>： | WSU-DLCL |
| DSMZ no. ： | TS260303 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1988 from the malignant pleural fluid of a 32-year-old woman with diffuse large B cell lymphoma at terminal stage |
| Reference(s) ： | 24246 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing mostly in clumps in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.2-0.3 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 3-4 days; cells start to proliferate after approximately one week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-72 hours |
| Harvest ： | maximum density at ca. 0.9 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD13 -, CD19 +, CD20 -, CD34 -, HLA-DR -, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |