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| 产品名称： | PG-EBV |
|---|---|
| 商品货号： | TS260305 |
| <strong>Cell line </strong>： | PG-EBV |
| DSMZ no. ： | TS260305 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B lymphoblastoid cells |
| Origin ： | normal B-cell derived lymphoblastoid cell line established in 1988 from a man with chronic lymphocytic leukemia (CLL) by immortalization with B95-8 derived EBV; autologous to cell line PGA-1 (DSM ACC 766); cell line is also known as PG/B95-8 |
| Reference(s) ： | 19170, 19171, 19172 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells, growing singly or in clusters in suspension |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 24-well plates; optimal split ratio 1:2 - 1:3 twice a week; maintain at ca. 0.7 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | maximum density at ca. 1-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed authentic STR DNA profile identical to PGA-1 |
| Cytogenetics ： | human near diploid karyotype; 46(43-46)<2n>XY, der(Y)t(Y;2)(qter;p14); sdl with -2; related to published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |