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| 产品名称： | NALM-33 |
|---|---|
| 商品货号： | TS260315 |
| <strong>Cell line </strong>： | NALM-33 |
| DSMZ no. ： | TS260315 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established in 1996 from the peripheral blood of a 72-year-old man with B cell precursor (BCP) acute lymphoblastic leukemia (ALL) at relapse |
| Reference(s) ： | 18127 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in 24-well plates, maintain at 1-1.5 x 10(6) cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximum density at ca. 2.5-3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile identical to sister cell line NALM-34 by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |