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| 产品名称： | BLN-7 |
|---|---|
| 商品货号： | TS260322 |
| <strong>Cell line </strong>： | BLN-7 |
| DSMZ no. ： | TS260322 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | glioblastoma |
| Origin ： | established in 2013 from an intraoperative glioblastoma specimen of a 72-year-old woman |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | neuronal-like cells growing adherently as single cells with tendency to form clusters; FBS-induced differentiation may be visible by predominant monolayer formation; image; image; image |
| Medium ： | Neurobasal medium + 0.5x B-27 supplement without vitamin A, 0.5x N-2 supplement, 20 ng/ml bFGF, 20 ng/ml EGF, 2 mM L-glutamine; omit FBS! |
| Subculture ： | seed out at ca. 1.0 x 106 cells/25 cm2 flask; split culture 1:2 to 1:4 once or twice a week; trypsin should not be used, but cells can be detached by tapping the flask or vigorous pipetting |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-5 days |
| Harvest ： | cell harvest of ca. 8-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 90% medium, 10% DMSO (no FBS added) |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |