微信小李
微信小章
产品简介
购买须知
| 产品名称： | JIH-5 |
|---|---|
| 商品货号： | TS260323 |
| <strong>Cell line </strong>： | JIH-5 |
| DSMZ no. ： | TS260323 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | acute biphenotypic leukemia |
| Origin ： | established in 2009 from leukemic bone marrow cells of a 21-year old woman with mixed-phenotype acute leukemia (MPAL) at second relapse; cells express typical antigens of myeloid as well as B-lymphoid lineages; cells were described in the literature to express EP300-ZNF384 fusion gene |
| Reference(s) ： | 18132 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension; image; image; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1.5 x 106 cells/well in 24 well plates, subsequently cells may be grown in flasks, optimal split ratio 1:1.5 - 1:2 every 5-7 days; after thawing, culture cells temporarily in 24-well plates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 5-7 days |
| Harvest ： | maximum density at ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 -, CD13 +, CD15 +, CD19 +, CD20 -, CD21 -, CD34 -, CD37 -, CD38 +, CD138 -, HLA-DR +; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Molec. Genetics ： | expression of fusion genes EP300-ZNF384 and CHD4-EP300 confirmed by RT-PCR |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |