微信小李
微信小章
产品简介
购买须知
| 产品名称： | JVE-015 |
|---|---|
| 商品货号： | TS260325 |
| <strong>Cell line </strong>： | JVE-015 |
| DSMZ no. ： | TS260325 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | colon carcinoma |
| Origin ： | established from the tissue of a primary malignant tumor from the colon sigmoideum of a 66-year old woman; cells were described in the literature to carry mutations in the TP53, K-RAS, APC, and PIK3CA genes |
| Reference(s) ： | 18128 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently as monolayer and in large colonies ; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 - 1:5 once or twice a week using trypsin/EDTA (wash twice with PBS and incubate with trypsin/EDTA for at least 15 min.) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage ： | 70% RPMI 1640 + 20% FBS + 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |