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| 产品名称： | ETCC-008 |
|---|---|
| 商品货号： | TS260333 |
| <strong>Cell line </strong>： | ETCC-008 |
| DSMZ no. ： | TS260333 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | breast ductal carcinoma |
| Origin ： | established in 2010 from the tumor tissue biopsy of a 47-year-old woman of Singapore Chinese origin with breast ductal carcinoma in situ (DCIS); cells were immortalized by stable transformation with human telomerase reverse transcriptase (hTERT); the ETCC-008 cell line is one of a series of five immortalized colonies from the same tumor sample (ETCC-006, ETCC-007, ETCC-008, ETCC-010, ETCC-011) |
| Reference(s) ： | 18123 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The cell line ETCC-008 was established by stable lipofectamin transformation of breast carcinoma cells with the pGRN145 plasmid coding for the catalytic subunit of the human telomerase reverse transcriptase (hTERT) and for hygromycin resistence. Donor cells, vector und recipient cells match risik group 1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:10 every four days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile identical to sister cell lines ETCC-006, ETCC-007, ETCC-010, and ETCC-011 by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |