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| 产品名称： | MBU-IM |
|---|---|
| 商品货号： | TS260339 |
| <strong>Cell line </strong>： | MBU-IM |
| DSMZ no. ： | TS260339 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | keratinocytes |
| Origin ： | established in 2011 from primary keratinocytes of a healthy neonatal foreskin; cells were immortalized by transduction with retroviral vectors containing human telomerase and SV40 early region |
| Reference(s) ： | 25648 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviruses containing a) the catalytic subunit of human telomerase reverse transcriptase hTERT, and b) SV40 early region for immortalization; donor, vector, and recipient cells are categorized to biosafety level 1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing in monolayers; image; image |
| Medium ： | 100% Keratinocyte SFM + 0.2ng/ml hEGF + 15µg/ml pituitary extract |
| Subculture ： | seed out at ca. 0.3 x 106 cells/80 cm2 flask; split 1:4 - 1:8 every 5-8 days using Accutase |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 2-3 x 106 cells/80 cm2 |
| Storage ： | frozen with 90% Keratinocyte SFM, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed the same STR DNA profile as MBU-T, MBU-TS1, and MBU-TS4 |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |