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| 产品名称： | U-2946 |
|---|---|
| 商品货号： | TS260344 |
| <strong>Cell line </strong>： | U-2946 |
| DSMZ no. ： | TS260344 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1996 from the pleural effusion of a 52-year-old man with diffuse large B cell lymphoma (DLBCL). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 18124, 18137, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in small clumps in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.1-0.3 x 106 cells/ml, optimal split ratio 1:5 - 1:10 every 3-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-30 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Cytogenetics ： | human hyperdiploid karyotype; -47(43-48)<2n>XY, +7, der(8)del(8)(p22.2)t(8:14)(q24;q32), der(9)t(2;9)(p25:p21), der(14)t(8;14)(q24;q32), der(17)del(17)(p11.2)del(17)(q21q22); sdl with del(9)(q11); carries t(8;14) effecting IGH-MYC rearrangement; der(17) carries 1q21-derived focal amplicon inserted at 17p11 effecting MCL1 activation |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |