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| 产品名称： | PCL-12 |
|---|---|
| 商品货号： | TS260346 |
| <strong>Cell line </strong>： | PCL-12 |
| DSMZ no. ： | TS260346 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 2012 from the peripheral blood of a 73 year-old man with chronic lymphocytic leukemia (CLL Rai stage I, Binet stage A) after multiple chemotherapeutic treatments; cells are described in the literature to highly express CD19 and CD5; however, DSMZ immunoprofiling showed that the level of CD5 expressing cells decrease to about 10% after long-term cultivation |
| Reference(s) ： | 18125 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot in phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a at least inducible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing in big clusters in suspension |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD 10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +; image |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |