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| 产品名称： | GL-261 |
|---|---|
| 商品货号： | TS260351 |
| <strong>Cell line </strong>： | GL-261 |
| DSMZ no. ： | TS260351 |
| Species ： | confirmed as mouse by species-PCR and COI barcoding; inbred strain analysis excluded DBA/s, Swiss, Balb/c, C3H and 129Sv. |
| Cell type ： | glioblastoma |
| Origin ： | established as in vitro growing cell culture in the mid 1990s from the Gl261 tumor which was induced in 1939 by intracranial injection of 3-methylcholanthrene into C57BL/6 mice and subsequently maintained by serial transplantation in syngeneic mouse strain; cells were described in the literature to carry TP53 and KRAS mutations |
| Reference(s) ： | 18129, 18130, 18131 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid cells growing adherently in monolayers; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + 2 mM glutamine (end concentration = 4 mM glutamine) |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2 flask; split 1:3 - 1:4 every 5-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 80-100 hours |
| Harvest ： | cell harvest of ca. 6 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |