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| 产品名称： | JVE-017 |
|---|---|
| 商品货号： | TS260354 |
| <strong>Cell line </strong>： | JVE-017 |
| DSMZ no. ： | TS260354 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | colon carcinoma |
| Origin ： | established from the tumor mass of a 67-year-old woman with colorectal cancer (cecum; Duke stage unknown) |
| Reference(s) ： | not published, for other cell lines of the series see, 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers or large clumps; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split culture 1:1 - 1:3 every 5-10 days (monolayer and aggregate populations appear to grow independently demanding care during passage to mitigate risk of selection); doubling time may be longer after recovery from liquid nitrogen |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |