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| 产品名称： | MBU-T |
|---|---|
| 商品货号： | TS260357 |
| <strong>Cell line </strong>： | MBU-T |
| DSMZ no. ： | TS260357 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | keratinocytes |
| Origin ： | established in 2011 from primary keratinocytes of a healthy neonatal foreskin; cells were immortalized by transduction with retroviral vectors containing human telomerase, SV40 early region, and epitope-tagged mutated HRAS(V12) linked to IRES-driven GFP expression |
| Reference(s) ： | 25648 |
| Biosafety level ： | 2, GMO-S2 |
| Risk assessment ： | Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviral vectors containing a) the catalytic subunit of the human telomerase reverse transcriptase hTERT, b) SV40 early region, and c) constitutively active mutated HRASV12 genes for immortalization. Mutated HRAS exhibits strong oncogenic potential and is assigned to biosafety level 2. |
| Permissions and restrictions ： | A, B, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing in monolayers; |
| Medium ： | 100% Keratinocyte SFM + 0.2 ng/ml hEGF + 15 µg/ml pituitary extract |
| Subculture ： | seed out at ca. 0.5 - 1 x 106 cells/80 cm2 flask; split 1:2 - 1:4 every 3-4 days using Accutase |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 4-6 x 106 cells/80 cm2 |
| Storage ： | frozen with 90% Keratinocyte SFM, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | fluorescent nonaplex PCR revealed the same STR DNA profile as MBU-IM, MBU-TS1, and MBU-TS4 |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |