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| 产品名称： | JVE-109 |
|---|---|
| 商品货号： | TS260374 |
| <strong>Cell line </strong>： | JVE-109 |
| DSMZ no. ： | TS260374 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon carcinoma |
| Origin ： | established from the tumor mass of an 84-year-old woman with colorectal adenocarcinoma (Dukes stage C1) from the hepatic flexure; cells were described in the literature to carry mutations in the BRAF and TP53 genes, hypermethylation of the MLH1 and CDKN2A promoters and to exhibit microsatellite instability (MSI-H) |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid adherent cells growing as monolayer and in densely packed islets; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:4 to 1:8 once or twice a week using trypsin/EDTA; cells may be difficult to disperse |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of 8-10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |