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| 产品名称： | CX-03 |
|---|---|
| 商品货号： | TS260385 |
| <strong>Cell line </strong>： | CX-03 |
| DSMZ no. ： | TS260385 |
| Species ： | human (Homo sapiens) |
| Cell type ： | uterine carcinosarcoma |
| Origin ： | established in 2015 after xenotransplantation in SCID-mice of primary tumor cells from a mixed Muellerian tumor (Carcinosarcoma) of the uterus of a 49-year-old Caucasian woman; cells are described in literature to produce mesothelin and mucus |
| Reference(s) ： | 18179 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers; image; image |
| Medium ： | 80% Dulbecco´s MEM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:2 -1:4 every 7 days using trypsin/EDTA; cells grow rather slowly in the first two week after thawing |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 4-7 days |
| Harvest ： | cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |