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| 产品名称： | MAPAC-HS-77 |
|---|---|
| 商品货号： | TS260386 |
| <strong>Cell line </strong>： | MAPAC-HS-77 |
| DSMZ no. ： | TS260386 |
| Species ： | human (Homo sapiens) |
| Cell type ： | pancreatic cancer |
| Origin ： | established in 2015 from the resection of the head of the pancreas of a 64-year-old man suffering from pancreatic tumor (TNM stage pT3, pN1, L0 V0 Pn1; tumor grade G3; resection status R0) |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers |
| Medium ： | 80% mixture of Hams F12 + Dulbeccos MEM high glucose (at 1:1) + 20% h.i. horse serum |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:5 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2 days |
| Harvest ： | cell harvest of ca. 30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |