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| 产品名称： | JVE-241 |
|---|---|
| 商品货号： | TS260393 |
| <strong>Cell line </strong>： | JVE-241 |
| DSMZ no. ： | TS260393 |
| Species ： | human |
| Cell type ： | colon carcinoma |
| Origin ： | established from the primary tissue of a mucinuous colon adenocarcinoma of the cecum (Dukes stage B2) of a 79-year-old man; cells were described in literature to carry mutations in the KRAS, APC and TP53 genes |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round and polymorphic cells growing in suspension with the tendency to pile up and formation of clusters; image; image; image |
| Medium ： | 80% mixture of Hams F12 + Dulbeccos MEM (1:1) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2; split growing culture 1:2 every 7-10 days; clusters may be difficult to count and to separate |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 8-10 days |
| Harvest ： | cell harvest of ca. 6 x 106 cells/175 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |