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| 产品名称： | IPH-926 |
|---|---|
| 商品货号： | TS260398 |
| <strong>Cell line </strong>： | IPH-926 |
| DSMZ no. ： | TS260398 |
| Species ： | human (Homo sapiens) |
| Cell type ： | breast carcinoma |
| Origin ： | established from malignant ascites of a metastatic lobular breast cancer of a 72-year-old woman after chemotherapy (tamoxifen) and radiotherapy; cells are described in literature to carry a CDH1 mutation (lack of E-Cadherin expression). Primary tumor cells were ER-positive but cell line is described to be triple-negative. Due to a mutation in the TP53 gene cells are temperature sensitive. Further described to overexpress the MDR1/ABCB1 drug transporter, to lack beta-catenin expression, to be hyperdiploid and tumorigenic in SCID mice |
| Reference(s) ： | 18153, 18154, 18155, 18156 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round and epithelioid cells growing adherently in monolayers and grape-like clusters; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS, containing additionally 10 mM HEPES, 2.5 g/l glucose, 2 mM L-glutamine, 1 mM pyruvate, 10 µg/ml insulin |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:4 to 1:8 every 5-10 days using trypsin/EDTA; due to the temperature sensitivity cells should be kept and handled whenever possible at 37°C; medium should be pre-warmed before use |
| Incubation ： | obligatory at 37 °C with 5% CO2 |
| Doubling time ： | in the first 1-2 weeks about 200 hours then 80-120 hours |
| Harvest ： | ca. 15-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |