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| 产品名称： | MCA-B1 |
|---|---|
| 商品货号： | TS260401 |
| <strong>Cell line </strong>： | MCA-B1 |
| DSMZ no. ： | TS260401 |
| Species ： | confirmed as Canis lupus origin by COI DNA barcoding |
| Cell type ： | acanthomatous epulis |
| Origin ： | odontogenic tumor of the gingiva (acanthomatous epulis); established in 1992 from the tumor tissue from a 10-year old domestic dog (Akita bred, Japan) |
| Reference(s) ： | 27645, 27648 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers with pavement-like colonies; image; image |
| Medium ： | mixture of Hams F12 + Dulbeccos MEM (1:1) + 5-10% h.i. FBS |
| Subculture ： | seed out 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of 6-8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Viruses ： | PCR: MLV - |