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| 产品名称： | JVE-528 |
|---|---|
| 商品货号： | TS260407 |
| <strong>Cell line </strong>： | JVE-528 |
| DSMZ no. ： | TS260407 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon carcinoma |
| Origin ： | established from the primary tissue of the ascending colon of a 57-year-old woman with malignant adenocarcinoma (Dukes stage B1); cells were described in literature to carry mutations in the KRAS, TP53 and APC genes and methylation of the MGMT promoter |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers and in densely packed islets, sometimes in multilayered clusters; note: freshly passaged cells need 3-4 days to adhere; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 2-3 x 106 cells/25 cm2; split confluent culture 1:2 once a week using trypsin/EDTA; however cells may need several days to adhere after thawing and trypsination; we recommend to use always a new culture flask after trypsination |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 4 days |
| Harvest ： | cell harvest of 12-14 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |