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| 产品名称： | EL-4 |
|---|---|
| 商品货号： | TS260416 |
| <strong>Cell line </strong>： | EL-4 |
| DSMZ no. ： | TS260416 |
| Species ： | confirmed as Mus musculus origin by COI DNA barcoding and by STR typing as strain C57BL/6N |
| Cell type ： | T cell lymphoma |
| Origin ： | established from a Dimethyl-benzanthracene induced lymphoma in a C57BL/6N mouse |
| Reference(s) ： | 18170, 18171 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, rounded to oval or multiform cells growing mostly singly in suspension; some cells exhibit tiny hairs or fringes; image; image |
| Medium ： | 95% RPMI 1640 + 5% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain between 1.0 and 1.5 x 106 cells/ml; split saturated culture 1:3-1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 15-20 hours |
| Harvest ： | maximal density at about 1.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Viruses ： | PCR: MLV + |