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| 产品名称： | U2OS#18-XRCC2-5E |
|---|---|
| 商品货号： | TS260423 |
| <strong>Cell line </strong>： | U2OS#18-XRCC2-5E |
| DSMZ no. ： | TS260423 |
| Species ： | human |
| Cell type ： | osteosarcoma |
| Origin ： | Genetical modification of U2OS#18 (DSMZ ACC 832, a reporter cell line for measurement of sister chromatid recombination, SCR) with a genetic disruption of XRCC2 by frameshifting mutations in exon 3 (targeted by CRISPR-CAS9; puromycin resistant and expressing GFP). The wild-type allele is no longer present. A sister cell line with the retrovirally reconstituted XRCC2 gene is available as ACC 841 |
| Reference(s) ： | 18169, 18186 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; image ; image |
| Medium ： | 80% Dulbecco´s MEM + 20% h.i. FBS + (additionally) 2 mM L-glutamine (to verify puromycin resistance, 1 µg/ml should be added) |
| Subculture ： | seed out at 1 x 106 cells/80 cm2; split confluent culture once a week 1:2 to 1:3 using trypsin/EDTA (wash twice with PBS before adding trypsin/EDTA); apparently confluent cultures can be further cultivated for 4-6 days to increase the maximal cell harvest |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 4-6 days |
| Harvest ： | cell harvest of ca. 4-8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed an authentic STR DNA profile as cell line U-2-OS (DSMZ ACC 785) |
| Molec. Genetics ： | frameshift mutations in exon 3 of XRCC2 were verified by sequence analysis |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |