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| 产品名称： | U2OS#18-RAD51B-8/B+ |
|---|---|
| 商品货号： | TS260427 |
| <strong>Cell line </strong>： | U2OS#18-RAD51B-8/B+ |
| DSMZ no. ： | TS260427 |
| Species ： | human |
| Cell type ： | osteosarcoma |
| Origin ： | Genetical modification of U2OS#18 (DSMZ ACC 832), a reporter cell line for studying sister chromatid recombination, SCR). This cell line belongs to the series of genetically disrupted RAD51 paralogs with reversion of the disruption by retroviral transfection with the functional gene (the non-complemented sister cell line is ACC 833). Cells are described in the literature to be puromycin and hygromycin resistant |
| Reference(s) ： | 18169, 18186 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to verify antibiotic resistance, 1 µg/ml puromycin and/or 150 µg/ml hygromycin should be added) |
| Subculture ： | seed out at 1 x 106 cells/80 cm2; split confluent culture 1:4 to 1:10 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 25-30 hours |
| Harvest ： | cell harvest of 10-12 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed an authentic STR DNA profile as cell line U-2-OS (DSMZ ACC 785) |
| Molec. Genetics ： | frameshift mutation in exon 3 of RAD51B was verified by sequence analysis and forced expression of RAD51B in the retrovirally transfected cell line was confirmed by RQ-PCR analysis |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |