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| 产品名称： | HEK293-DR-GFP-RAD51D-16 |
|---|---|
| 商品货号： | TS260442 |
| <strong>Cell line </strong>： | HEK293-DR-GFP-RAD51D-16 |
| DSMZ no. ： | TS260442 |
| Species ： | human |
| Cell type ： | embryonal kidney |
| Origin ： | Genetic modification of HEK293-DR-GFP (DSMZ ACC 844, a hprtDR-GFP reporter cell line for studying chromosomal double-strand repair (DR)). This cell line carries a genetic disruption of RAD51D by frameshifting mutations in exon 1 (targeted by CRISPR-CAS9) and is puromycin resistant. The wild-type allele is no longer present. A sister cell line with the retrovirally reconstituted RAD51D gene is available as ACC 852 |
| Reference(s) ： | 18173, 18186 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayer; image; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to sustain genetic stability cells should be maintained, at least periodically, with 0.5 µg/ml puromycin) |
| Subculture ： | culture vessels should be coated with 0.01% poly-L-lysine solution; seed out at 0.5-1 x 106 cells/80 cm2; split subconfluent culture 1:2 to 1:4 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed an authentic STR DNA profile matching parental cell line (HEK)293 (DSMZ ACC 305) |
| Molec. Genetics ： | frameshift mutation in exon 1 of RAD51D was verified by sequence analysis |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, XMLV - |