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| 产品名称： | HEK293-DR-GFP-XRCC2-13/X2+ |
|---|---|
| 商品货号： | TS260452 |
| <strong>Cell line </strong>： | HEK293-DR-GFP-XRCC2-13/X2+ |
| DSMZ no. ： | TS260452 |
| Species ： | human |
| Cell type ： | embryonal kidney |
| Origin ： | Genetic modification of HEK293-DR-GFP-XRCC2-13 (DSMZ ACC 848) which belongs to a series of genetically disrupted RAD51 paralogs. Here, in TS260452, the XRCC2 gene is functionally reconstituted by retroviral transfection. Cells are described in the literature to be puromycin and hygromycin resistant |
| Reference(s) ： | 18186, 18173 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayer; image; image; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to sustain genetic stability cells should be maintained, at least periodically, with 0.5 µg/ml puromycin and/or 100 µg/ml hygromycin) |
| Subculture ： | culture vessels should be coated with 0.01% poly-L-lysine solution; seed out at 1 x 106 cells/80 cm2; split 1:5 to 1:10 once or twice a week using TrypleExpress (trypsin/EDTA may not be efficient) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 20 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed an authentic STR DNA profile matching parental cell line (HEK)293 (DSMZ ACC 305) |
| Molec. Genetics ： | frameshift mutation in exon 3 of XRCC2 was verified by sequence analysis and forced expression of XRCC2 in the retrovirally transfected cell line was confirmed by RQ-PCR analysis |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, XMLV - |