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| 产品名称： | N1E-115-1 |
|---|---|
| 商品货号： | TS260459 |
| <strong>Cell line </strong>： | N1E-115-1 |
| DSMZ no. ： | TS260459 |
| Species ： | confirmed as Mus musculus origin by COI DNA barcoding |
| Cell type ： | neuroblastoma |
| Origin ： | Subclone of N1E-115, an adrenergic cell line established from mouse strain A/Jax with neuroblastoma C1300 |
| Reference(s) ： | 14354, 18176 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round adherent cells growing in monolayers or small clumps, occasionally forming spindle-like extensions; image ; image ; image |
| Medium ： | 80-90% Dulbeccos MEM (with 25 mM HEPES) + 10-20% h.i. FBS |
| Subculture ： | seed out at 0.25 x 106 cells/25 cm2; viability is rather low after thawing, but cells recover soon, when medium is exchanged every second day; culture does not grow to complete confluency; split sub confluent culture 1:2-1:4 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Viruses ： | PCR: XMLV+ |