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| 产品名称： | MUG-MEL2 |
|---|---|
| 商品货号： | TS260467 |
| <strong>Cell line </strong>： | MUG-MEL2 |
| DSMZ no. ： | TS260467 |
| Species ： | human |
| Cell type ： | melanoma |
| Origin ： | established in 2014 from a cutaneous metastasis of a 48-year-old man with highly aggressive ulcerated melanoma after chemotherapy. Cells are described to be moderately hyperdiploid, carry a mutation in the N-Ras-gene (pQ61R) but not in the BRAF. Further described to be tumorigenic in nude mice and able to grow in 3D using hydrogel |
| Reference(s) ： | 18175 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | elongate or triangular dendritic cells growing adherently as monolayer; cells are highly pigmented and sedimented cells have a brown color; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM glutamine |
| Subculture ： | seed out at 1 x 106 cells/80 cm2; split confluent culture 1:4 to 1:6 every 4-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | fluorescent nonaplex PCR revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, XMLV - |