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| 产品名称： | UPCI-SCC-003 |
|---|---|
| 商品货号： | TS260471 |
| <strong>Cell line </strong>： | UPCI-SCC-003 |
| DSMZ no. ： | TS260471 |
| Species ： | human |
| Cell type ： | squamous cell carcinoma |
| Origin ： | established in 1992 from a new primary tumor of the tonsils (grade 1, T1N0) of a 65-year-old woman with head and neck squamous cell carcinoma. The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-414-648-4004) at the University of Pittsburgh, Office of Technology Management |
| Reference(s) ： | 15061, 14735, 15176 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers; image; image |
| Medium ： | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine + 1% sodium pyruvate +1% non-essential amino acids |
| Subculture ： | seed out at about 1 x 106 cells/80 cm2; split semi-confluent culture up to 1:10 once a week using TripleExpress. Flasks coated with 0.01% poly-L-lysine may be used if the cells do not adhere |
| Incubation ： | at 37 °C with 5-10% CO2 |
| Doubling time ： | ca. 45 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to make inquiries about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, XMLV - |