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| 产品名称： | INA-6 |
|---|---|
| 商品货号： | TS260473 |
| <strong>Cell line </strong>： | INA-6 |
| DSMZ no. ： | TS260473 |
| Species ： | human |
| Cell type ： | multiple myeloma |
| Origin ： | established from the pleural effusion of an 80-year old patient with plasma cell leukemia in 1988. Cell line is IL-6 dependent and was described in the literature to carry an activating mutation of the N-RAS gene and del(1)(p22), del(7)(p13) and 14q+: t(11;14)(q13;q32) translocation |
| Reference(s) ： | 18191, 18192, 18193, 18194 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells of different sizes, growing singly or in small clumps in suspension; image;image |
| Medium ： | 80% RPMI 1640 + 20% FBS + 10 ng/ml IL-6 |
| Subculture ： | seed out 0.5-1.0 x 106 cells/well in a 24-well plate or at 0.5 x 106 cells/ml in a small culture flask; maintain at 0.5-1.5 x 106 cells/ml, split 1:2 to 1:4 every 2-3 days. After thawing growth may be delayed |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-50 hours |
| Harvest ： | maximal density of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 -, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, smkappa -, cykappa +, sm/cylambda -; image |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, XMLV - |