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| 产品名称： | FAIK3-5 |
|---|---|
| 商品货号： | TS260476 |
| <strong>Cell line </strong>： | FAIK3-5 |
| DSMZ no. ： | TS260476 |
| Species ： | confirmed as Mus musculus origin by COI DNA barcoding. STR profile is consistent with STR data of mouse C57/BL6 |
| Cell type ： | kidney |
| Origin ： | REP (renal Epo-producing) cells from the kidney of adult transgenic female mice (B6D2;C57BL6N-Tg(EPO::Cre)1RHW x
B6.Cg-Gt(ROSA)26Sor |
| Reference(s) ： | 18189 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | flat, partially round, spindle and polygonal (fibroblastoid, telocyte-like) cells growing adherently in monolayers; image; image |
| Medium ： | 90-95% Dulbecco´s MEM + 5-10% h.i. FBS |
| Subculture ： | seed out 0.5-1 x 106 cells/80 cm2; split 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2, normal oxygen level |
| Doubling time ： | about 20-30 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Viruses ： | PCR: XMLV - |