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| 产品名称： | JK-6 |
|---|---|
| 商品货号： | TS260477 |
| <strong>Cell line </strong>： | JK-6 |
| DSMZ no. ： | TS260477 |
| Species ： | human |
| Cell type ： | multiple myeloma |
| Origin ： | established from peripheral blood of a 75-year-old male with multiple myeloma in 1992. Cells were originally IL-6 dependent but became independent during the further course of establishment. Described to carry mutations in the K- and N-RAS genes. More cytogenetic and immunological data, including HLA types are available upon request to mutz@dsmz.de |
| Reference(s) ： | 18188 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round (plasmablasts-like) cells growing in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% FBS + 2 mM L-glutamine |
| Subculture ： | seed out at about 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 48 hours |
| Harvest ： | maximal density of ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, XMLV - |