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| 产品名称： | UPCI-SCC-081 |
|---|---|
| 商品货号： | TS260480 |
| <strong>Cell line </strong>： | UPCI-SCC-081 |
| DSMZ no. ： | TS260480 |
| Species ： | human |
| Cell type ： | oral squamous cell carcinoma |
| Origin ： | established in 1994 from a primary tumor tissue of a squamous cell carcinoma (stage T4N0) of the alveolar ridge of a 87-year-old woman. Described to carry a chromosomal 11q13 amplification and some additional alterations. The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-414-648-4004) at the University of Pittsburgh, Office of Technology Management |
| Reference(s) ： | 14735, 15061, 18200 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small epithelioid cells growing adherently as monolayer; image; image; image |
| Medium ： | 80% MEM (with Earles salts) + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at about 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA (wash 2x with PBS and then incubate for up to 10 min with trypsin) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 8-10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in PCR assay |
| Immunology ： | to make inquiries about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, XMLV - |